pBABEneo-HRASV12 | Addgene 71304 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pBABEneo-HRASV12

catalog :

71304

citations: 1

Reference
Lock R, Roy S, Kenific C, Su J, Salas E, Ronen S, et al. Autophagy facilitates glycolysis during Ras-mediated oncogenic transformation. Mol Biol Cell. 2011;22:165-78 pubmed publisher

product information

Catalog Number :

71304

Product Name :

pBABEneo-HRASV12

article :

doi	10.1091/mbc.E10-06-0500
id	16056
pubmed_id	21119005

bacterial resistance :

Ampicillin

cloning :

backbone

pBABE-neo

backbone_mutation

backbone_origin

backbone_size

5300

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

The insert within this plasmid contains a E143K mutation compared to NP_005334.1. The depositor states that this mutation should not affect plasmid function. The HRAS coding sequence may have been codon optimized and differs from the NCBI reference sequences for this gene. Primer design and applications involving the use of the HRAS coding sequence should be based off of the Addgene NGS Result for accuracy

growth strain :

Retroviral expression of H-RAS-V12

origin :

pi :

alt_names

HRAS

HRASV12

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	BamHI
promoter
sequencing_primer_3	pBABE 3'
sequencing_primer_5	pBABE 5'
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras
gene	HRAS
id	3265

genbank_ids

mutation

G12V mutation

name

H-RAS-V12

shRNA_sequence

size

species

9606

Homo sapiens