This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA GASTM-3-RVG-10-Lamp2b-HA
catalog :
71295
citations: 1
Reference
Hung M, Leonard J. Stabilization of exosome-targeting peptides via engineered glycosylation. J Biol Chem. 2015;290:8166-72 pubmed publisher
product information
Catalog Number :
71295
Product Name :
pcDNA GASTM-3-RVG-10-Lamp2b-HA
article :
doi10.1074/jbc.M114.621383
id16042
pubmed_id25657008
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA 3.1+ Hygro
backbone_mutationsome restriction sites removed from MCS
backbone_originClontech
backbone_size5578
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Encodes (N to C): GASTM mutated glycosylation motif, 3 residue spacer, RVG peptide,10 residue spacer, Lamp2b (exosomal membrane protein), 3 residue spacer, HA tag.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5NheI
promoterCMV
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesCD107b, DND, LAMP-2, LAMPB, LGP-96, LGP110
geneLAMP2
id3920
genbank_ids
mutation
nameLamp2b
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagLamp2 signal peptide
locationN terminal on insert
tagGASTM mutated glycosylation motif
locationN terminal on insert
tagRabies Viral Glycoprotein (RVG) peptide targeting acetycholine receptor
locationC terminal on insert
tagHA
plasmid copy :
Lamp2b cDNA was purchased from Open Biosystems
resistance markers :
2081
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA