This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA GNSTM-3-RVG-10-Lamp2b-HA
catalog :
71294
citations: 7
Reference
Li B, Xi W, Bai Y, Liu X, Zhang Y, Li L, et al. FTO-dependent m6A modification of Plpp3 in circSCMH1-regulated vascular repair and functional recovery following stroke. Nat Commun. 2023;14:489 pubmed publisher
Li B, Chen X, Qiu W, Zhao R, Duan J, Zhang S, et al. Synchronous Disintegration of Ferroptosis Defense Axis via Engineered Exosome-Conjugated Magnetic Nanoparticles for Glioblastoma Therapy. Adv Sci (Weinh). 2022;9:e2105451 pubmed publisher
Yu X, Bai Y, Han B, Ju M, Tang T, Shen L, et al. Extracellular vesicle-mediated delivery of circDYM alleviates CUS-induced depressive-like behaviours. J Extracell Vesicles. 2022;11:e12185 pubmed publisher
Zhang R, Fu Y, Cheng M, Ma W, Zheng N, Wang Y, et al. sEVsRVG selectively delivers antiviral siRNA to fetus brain, inhibits ZIKV infection and mitigates ZIKV-induced microcephaly in mouse model. Mol Ther. 2021;: pubmed publisher
Kim M, Kim G, Hwang D, Lee M. Delivery of High Mobility Group Box-1 siRNA Using Brain-Targeting Exosomes for Ischemic Stroke Therapy. J Biomed Nanotechnol. 2019;15:2401-2412 pubmed publisher
Kojima R, Bojar D, Rizzi G, Hamri G, El Baba M, Saxena P, et al. Designer exosomes produced by implanted cells intracerebrally deliver therapeutic cargo for Parkinson's disease treatment. Nat Commun. 2018;9:1305 pubmed publisher
Hung M, Leonard J. Stabilization of exosome-targeting peptides via engineered glycosylation. J Biol Chem. 2015;290:8166-72 pubmed publisher
product information
Catalog Number :
71294
Product Name :
pcDNA GNSTM-3-RVG-10-Lamp2b-HA
article :
doi10.1074/jbc.M114.621383
id16042
pubmed_id25657008
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA 3.1+ Hygro
backbone_mutationsome restriction sites removed from MCS
backbone_originClontech
backbone_size5578
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, RVG peptide, 10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5NheI
promoterCMV
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesCD107b, DND, LAMP-2, LAMPB, LGP-96, LGP110
geneLAMP2
id3920
genbank_ids
mutation
nameLamp2b
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagLamp2 signal peptide
locationN terminal on insert
tagGNSTM glycosylation motif
locationN terminal on insert
tagRabies Viral Glycoprotein (RVG) peptide targeting acetycholine receptor
locationC terminal on insert
tagHA
plasmid copy :
Lamp2b cDNA was purchased from Open Biosystems
resistance markers :
2081
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA