This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA GNSTM-3-Flag-10-Lamp2b-HA
catalog :
71293
citations: 3
Reference
Kim H, Mun D, Kang J, Lee S, Yun N, Joung B. Improved cardiac-specific delivery of RAGE siRNA within small extracellular vesicles engineered to express intense cardiac targeting peptide attenuates myocarditis. Mol Ther Nucleic Acids. 2021;24:1024-1032 pubmed publisher
Kim H, Yun N, Mun D, Kang J, Lee S, Park H, et al. Cardiac-specific delivery by cardiac tissue-targeting peptide-expressing exosomes. Biochem Biophys Res Commun. 2018;499:803-808 pubmed publisher
Hung M, Leonard J. Stabilization of exosome-targeting peptides via engineered glycosylation. J Biol Chem. 2015;290:8166-72 pubmed publisher
product information
Catalog Number :
71293
Product Name :
pcDNA GNSTM-3-Flag-10-Lamp2b-HA
article :
doi10.1074/jbc.M114.621383
id16042
pubmed_id25657008
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA 3.1+ Hygro
backbone_mutationsome restriction sites removed from MCS
backbone_originClontech
backbone_size5578
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, Flag tag,10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5NheI
promoterCMV
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesCD107b, DND, LAMP-2, LAMPB, LGP-96, LGP110
geneLAMP2
id3920
genbank_ids
mutation
nameLamp2b
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagLamp2 signal peptide
locationN terminal on insert
tagGNSTM glycosylation motif
locationN terminal on insert
tagFLAG
locationC terminal on insert
tagHA
plasmid copy :
Lamp2b cDNA was purchased from Open Biosystems
resistance markers :
2081
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA