pAP1-3 | Addgene 71258 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pAP1-3

catalog :

71258

citations: 1

Reference
Hay E, Cowie P, McEwan A, Ross R, Pertwee R, Mackenzie A. Disease-associated polymorphisms within the conserved ECR1 enhancer differentially regulate the tissue-specific activity of the cannabinoid-1 receptor gene promoter; implications for cannabinoid pharmacogenetics. Hum Mutat. 2020;41:291-298 pubmed publisher

product information

Catalog Number :

71258

Product Name :

pAP1-3

article :

doi
id	16029
pubmed_id

bacterial resistance :

Ampicillin

cloning :

backbone

pGM55

backbone_mutation

backbone_origin

Cockerill lab, Addgene plasmid 71249

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Luciferase

growth notes :

Reference for AP-1 site = Cockerill PN, Shannon MF, Bert AG, Ryan GR and Vadas MA. The GM-CSF/IL3 locus is regulated by an inducible cyclosporin A sensitive enhancer. Proc.Natl.Acad.Sci. USA 90, 2466-2470, 1993. JM109 are the cells used by the author for pXPG and its derivatives.
AGATCTGGATCACCCGCAGCTTGACTCATCCTTGCAGAT
CT
Cloned as 3 copies of an oligonucleotide duplex with complementary overhanging BglII ends:

growth strain :

luciferase reporter with 3 copies of the stromelylisin gene AP-1 site in front of hGM-CSF -55 to +28 minimal promoter

origin :

pi :

alt_names

matrix metallopeptidase 3

cloning

clone_method	Restriction Enzyme
cloning_site_3	SmaI
cloning_site_5	XhoI
promoter	GM-CSF minimal
sequencing_primer_3	LucNRev
sequencing_primer_5	unknown
site_3_destroyed	1
site_5_destroyed

entrez_gene

aliases	CHDS6, MMP-3, SL-1, STMY, STMY1, STR1
gene	MMP3
id	4314

genbank_ids

mutation

name

3 copies of an AP-1 site from the human Stromelysin-1 gene

shRNA_sequence

size

species

9606

Homo sapiens