This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
p420-3
catalog :
71257
citations: 1
Reference
Johnson B, Bert A, Ryan G, Condina A, Cockerill P. Granulocyte-macrophage colony-stimulating factor enhancer activation requires cooperation between NFAT and AP-1 elements and is associated with extensive nucleosome reorganization. Mol Cell Biol. 2004;24:7914-30 pubmed
product information
Catalog Number :
71257
Product Name :
p420-3
article :
doi10.1128/MCB.24.18.7914-7930.2004
id16028
pubmed_id15340054
bacterial resistance :
Ampicillin
cloning :
backbonepGM55
backbone_mutation
backbone_originCockerill lab, Addgene plasmid 71249
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Luciferase
growth notes :
JM109 are the cells used by the author for pXPG and its derivatives.
growth strain :
luciferase reporter with 3 copies of the GM420 NFAT-AP-1 site in front of hGM-CSF -55 to +28 minimal promoter
origin :
37
pi :
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterGM-CSF minimal
sequencing_primer_3LucNRev
sequencing_primer_5unknown
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCSF, GMCSF
geneCSF2
id1437
genbank_ids
mutation
name3 copies of the GM420 NFAT-AP-1 site
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagluciferase
resistance markers :
2616
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA