pGME | Addgene 71252 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pGME

catalog :

71252

citations: 1

Reference
Cockerill P, Bert A, Roberts D, Vadas M. The human granulocyte-macrophage colony-stimulating factor gene is autonomously regulated in vivo by an inducible tissue-specific enhancer. Proc Natl Acad Sci U S A. 1999;96:15097-102 pubmed

product information

Catalog Number :

71252

Product Name :

pGME

article :

doi
id	16025
pubmed_id	10611344

bacterial resistance :

Ampicillin

cloning :

backbone

pGM

backbone_mutation

backbone_origin

Cockerill lab, Addgene plasmid 71250

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Luciferase

growth notes :

Note that the 717-bp GM-CSF enhancer contains an extra G at position 118 from the sequence published in Cockerill et al. Proc Natl Acad Sci USA. 1993;90:2466 2470. JM109 are the cells used by the author for pXPG and its derivatives.

growth strain :

luciferase reporter with GM-CSF promoter + 717 bp Bgl II GM-CSF enhancer

origin :

pi :

alt_names

colony stimulating factor 2 (granulocyte-macrophage)

cloning

clone_method	Restriction Enzyme
cloning_site_3	BglII
cloning_site_5	BglII
promoter	GM-CSF
sequencing_primer_3	LucNRev
sequencing_primer_5	unknown
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CSF, GMCSF
gene	CSF2
id	1437

genbank_ids

mutation

name

GM-CSF enhancer

shRNA_sequence

size

species

9606

Homo sapiens