pGM | Addgene 71250 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pGM

catalog :

71250

citations: 2

Reference
Wang X, Lennard Richard M, Li P, Henry B, Schutt S, Yu X, et al. Expression of GM-CSF Is Regulated by Fli-1 Transcription Factor, a Potential Drug Target. J Immunol. 2021;206:59-66 pubmed publisher
Cockerill P, Bert A, Roberts D, Vadas M. The human granulocyte-macrophage colony-stimulating factor gene is autonomously regulated in vivo by an inducible tissue-specific enhancer. Proc Natl Acad Sci U S A. 1999;96:15097-102 pubmed

product information

Catalog Number :

71250

Product Name :

pGM

article :

doi
id	16025
pubmed_id	10611344

bacterial resistance :

Ampicillin

cloning :

backbone

pXPG

backbone_mutation

backbone_origin

Cockerill lab, Addgene plasmid 71248

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Luciferase

growth notes :

Before being inserted into pXPG, the GM-CSF promoter has been through successive rounds of recloning which have added cloning sites to the ends. JM109 are the cells used by the author for pXPG and its derivatives.

growth strain :

luciferase reporter with human GM-CSF promoter -627 to + 28

origin :

pi :

alt_names

colony stimulating factor 2 (granulocyte-macrophage)

cloning

clone_method	Restriction Enzyme
cloning_site_3	HindIII
cloning_site_5	HindIII
promoter	GM-CSF
sequencing_primer_3	LucNRev
sequencing_primer_5	unknown
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CSF, GMCSF
gene	CSF2
id	1437

genbank_ids

mutation

name

GM-CSF promoter -627 to +28 fragment

shRNA_sequence

size

species

9606

Homo sapiens