This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLV-tetO-Ascl2
catalog :
71151
citations: 1
product information
Catalog Number :
71151
Product Name :
pLV-tetO-Ascl2
article :
| doi | 10.1016/j.stem.2015.08.005 |
| id | 15954 |
| pubmed_id | 26412560 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pLV-tetO | ||
| backbone_mutation | |||
| backbone_origin | Hochedlinger Lab (Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.) | ||
| backbone_size | 8370 | ||
| promoter | |||
| sequencing_primer_3 | |||
| sequencing_primer_5 | |||
| vector_types |
|
growth notes :
The cDNA sequence of murine Ascl2 was amplified and subcloned into the pCR2.1 vector. From there it was excised via flanking EcoRI sites and inserted into the pLV-tetO vector via the EcoRI. The pLV-tetO vector backbone was obtained through EcoRI mediated excision of Oct4 cDNA from pLV-tetO-Oct4 (Stadtfeld et al., 2008, addgene Plasmid #19766), kindly provided by K. Hochedlinger (Harvard University).
growth strain :
Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Ascl2/Mash2
origin :
37
pi :
|
resistance markers :
| 2612 |
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
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