This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDEST-FRT/T0-Flag-BRCA1
catalog :
71117
citations: 2
Reference
Batenburg N, Walker J, Coulombe Y, Sherker A, Masson J, Zhu X. CSB interacts with BRCA1 in late S/G2 to promote MRN- and CtIP-mediated DNA end resection. Nucleic Acids Res. 2019;47:10678-10692 pubmed publisher
Orthwein A, Noordermeer S, Wilson M, Landry S, Enchev R, Sherker A, et al. A mechanism for the suppression of homologous recombination in G1 cells. Nature. 2015;528:422-6 pubmed publisher
product information
Catalog Number :
71117
Product Name :
pDEST-FRT/T0-Flag-BRCA1
article :
doi10.1038/nature16142
id16365
pubmed_id26649820
bacterial resistance :
Ampicillin
cloning :
backbonepDEST-Flag
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
siRNA resistant sequence: CAGCTACCCTagtATaATc The BRCA1 insert contains the following mutations (residue numbering relative to the plasmid's open reading frame): P1565L, E1732G, A1836T, K1877R, and S2307G
growth strain :
mammalian expression of Flag-BRCA1 siRNA resistant construct
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterCMV/TO
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53
geneBRCA1
id672
genbank_ids
mutation
nameBRCA1
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFlag
resistance markers :
2121
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA