This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDEST-FRT/T0-GFP-BRCA1
catalog :
71116
citations: 10
Reference
Wang J, Li L, Hu A, Deng G, Wei J, Li Y, et al. Inhibition of ASGR1 decreases lipid levels by promoting cholesterol excretion. Nature. 2022;608:413-420 pubmed publisher
Mazzucco G, Huda A, Galli M, Zanella E, Doksani Y. Purification of mammalian telomeric DNA for single-molecule analysis. Nat Protoc. 2022;17:1444-1467 pubmed publisher
Loke J, Alim I, Yam S, Klugman S, Xia L, Gruber D, et al. Prediction of breast cancer risk based on flow variant analysis of circulating peripheral blood mononuclear cells. HGG Adv. 2022;3:100085 pubmed publisher
Bashir S, Dang T, Rossius J, Wolf J, Kühn R. Enhancement of CRISPR-Cas9 induced precise gene editing by targeting histone H2A-K15 ubiquitination. BMC Biotechnol. 2020;20:57 pubmed publisher
Batenburg N, Walker J, Coulombe Y, Sherker A, Masson J, Zhu X. CSB interacts with BRCA1 in late S/G2 to promote MRN- and CtIP-mediated DNA end resection. Nucleic Acids Res. 2019;47:10678-10692 pubmed publisher
Svobodová Kovaříková A, Legartova S, Krejci J, Bartova E. H3K9me3 and H4K20me3 represent the epigenetic landscape for 53BP1 binding to DNA lesions. Aging (Albany NY). 2018;10:2585-2605 pubmed publisher
Park J, Lee S, Won N, Shin E, Kim S, Chun M, et al. Single-molecule DNA visualization using AT-specific red and non-specific green DNA-binding fluorescent proteins. Analyst. 2019;144:921-927 pubmed publisher
Xia Y, Ivanovska I, Zhu K, Smith L, Irianto J, Pfeifer C, et al. Nuclear rupture at sites of high curvature compromises retention of DNA repair factors. J Cell Biol. 2018;217:3796-3808 pubmed publisher
Moruno Manchon J, Koellhoffer E, Gopakumar J, Hambarde S, Kim N, McCullough L, et al. The G-quadruplex DNA stabilizing drug pyridostatin promotes DNA damage and downregulates transcription of Brca1 in neurons. Aging (Albany NY). 2017;9:1957-1970 pubmed publisher
Orthwein A, Noordermeer S, Wilson M, Landry S, Enchev R, Sherker A, et al. A mechanism for the suppression of homologous recombination in G1 cells. Nature. 2015;528:422-6 pubmed publisher
product information
Catalog Number :
71116
Product Name :
pDEST-FRT/T0-GFP-BRCA1
article :
doi10.1038/nature16142
id16365
pubmed_id26649820
bacterial resistance :
Ampicillin
cloning :
backbonepDEST-GFP
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
siRNA resistant sequence: CAGCTACCCTagtATaATc The BRCA1 insert contains the following mutations (residue numbering relative to the plasmid's open reading frame): P1565L, E1732G, A1836T, K1877R, and S2307G
growth strain :
mammalian expression of GFP-BRCA1 siRNA resistant construct
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterCMV/TO
sequencing_primer_3BGH-rev
sequencing_primer_5EGFP-C
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53
geneBRCA1
id672
genbank_ids
mutation
nameBRCA1
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagGFP
resistance markers :
2121
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA