This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDEST-mCherry-LacR-BRCA1
catalog :
71115
citations: 4
Reference
Hovland H, Mchaina E, H xf8 berg Vetti H, Ariansen S, Sjursen W, Van Ghelue M, et al. Functional Analyses of Rare Germline Missense BRCA1 Variants Located within and outside Protein Domains with Known Functions. Genes (Basel). 2023;14: pubmed publisher
Nath S, Nagaraju G. FANCJ helicase promotes DNA end resection by facilitating CtIP recruitment to DNA double-strand breaks. PLoS Genet. 2020;16:e1008701 pubmed publisher
Batenburg N, Walker J, Coulombe Y, Sherker A, Masson J, Zhu X. CSB interacts with BRCA1 in late S/G2 to promote MRN- and CtIP-mediated DNA end resection. Nucleic Acids Res. 2019;47:10678-10692 pubmed publisher
Orthwein A, Noordermeer S, Wilson M, Landry S, Enchev R, Sherker A, et al. A mechanism for the suppression of homologous recombination in G1 cells. Nature. 2015;528:422-6 pubmed publisher
product information
Catalog Number :
71115
Product Name :
pDEST-mCherry-LacR-BRCA1
article :
doi10.1038/nature16142
id16365
pubmed_id26649820
bacterial resistance :
Ampicillin
cloning :
backbonemCherry-LacR
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
siRNA resistant sequence: CAGCTACCCTagtATaATc The BRCA1 insert contains the following mutations (residue numbering relative to the plasmid's open reading frame): P1565L, E1732G, A1836T, K1877R, and S2307G
growth strain :
mammalian expression of mCherry-LacR-BRCA1 siRNA resistant construct
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterCMV/TO
sequencing_primer_3
sequencing_primer_5mCherry-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53
geneBRCA1
id672
genbank_ids
mutation
nameBRCA1
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagmCherry
resistance markers :
2121
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA