pLV-tetO-Elf5 | Addgene 70772 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLV-tetO-Elf5

catalog :

70772

citations: 1

Reference
Kubaczka C, Senner C, Cierlitza M, AraÃºzo Bravo M, Kuckenberg P, Peitz M, et al. Direct Induction of Trophoblast Stem Cells from Murine Fibroblasts. Cell Stem Cell. 2015;17:557-68 pubmed publisher

product information

Catalog Number :

70772

Product Name :

pLV-tetO-Elf5

article :

doi	10.1016/j.stem.2015.08.005
id	15954
pubmed_id	26412560

bacterial resistance :

Ampicillin

cloning :

backbone

pLV-tetO

backbone_mutation

backbone_origin

Hochedlinger Lab (Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.)

backbone_size

8370

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

The cDNA sequence of murine Elf5 was amplified from trophoblast stem cell cDNA and subcloned into the pCR2.1 vector. From there it was excised via BamHI and EcoRV and inserted into the pLV-tetO vector via the EcoRI site after Klenow treatment of both vector backbone and insert. The pLV-tetO vector backbone was obtained through EcoRI mediated excision of Oct4 cDNA from pLV-tetO-Oct4 (Stadtfeld et al., 2008, addgene Plasmid #19766), kindly provided by K. Hochedlinger (Harvard University).

growth strain :

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Elf5

origin :

pi :

alt_names

E74-like factor 5

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	EcoRI
promoter	tetO
sequencing_primer_3	WPRE-R: CATAGCGTAAAAGGAGCAACA
sequencing_primer_5	CMV-Promoter F: ACGCCATCCACGCTGTTTTGACCT
site_3_destroyed	1
site_5_destroyed	1

entrez_gene

aliases	ESE-2, ESE-5, ESE-5.
gene	Elf5
id	13711

genbank_ids

mutation

name

Elf5

shRNA_sequence

size

858

species

10090

Mus musculus