pAAV EF1a DIO iChloC 2A dsRed | Addgene 70762 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pAAV EF1a DIO iChloC 2A dsRed

catalog :

70762

citations: 2

Reference
Evans D, Stempel A, Vale R, Ruehle S, Lefler Y, Branco T. A synaptic threshold mechanism for computing escape decisions. Nature. 2018;558:590-594 pubmed publisher
Wietek J, Beltramo R, Scanziani M, Hegemann P, Oertner T, Wiegert J. An improved chloride-conducting channelrhodopsin for light-induced inhibition of neuronal activity in vivo. Sci Rep. 2015;5:14807 pubmed publisher

product information

Catalog Number :

70762

Product Name :

pAAV EF1a DIO iChloC 2A dsRed

article :

doi	10.1038/srep14807
id	15990
pubmed_id	26443033

bacterial resistance :

Ampicillin

cloning :

backbone

pAAV

backbone_mutation

backbone_origin

backbone_size

5601

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

AAV

growth strain :

An improved chloride-conducting channelrhodopsin for light-induced inhibition of neuronal activity in vivo. Cre inducible expression (double floxed inversed ORF)

origin :

pi :

alt_names

ChR2, iChloC

cloning

clone_method	Restriction Enzyme
cloning_site_3	NheI
cloning_site_5	KpnI
promoter	EF1a
sequencing_primer_3	GTAATCCAGAGGTTGATTATCG
sequencing_primer_5	GGGATTCTCCTCCACGTCACCGC
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

E83Q,E90R,E101S,D156N,T159C

name

Channelrhodopsin-2

shRNA_sequence

size

927

species

100

Synthetic

32644

Other

Chlamydomonas