This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET28HIS-hAPE1
catalog :
70757
citations: 4
Reference
Fleming A, Tran R, Omaga C, Howpay Manage S, Burrows C, Conboy J. Second Harmonic Generation Interrogation of the Endonuclease APE1 Binding Interaction with G-Quadruplex DNA. Anal Chem. 2022;94:15027-15032 pubmed publisher
Li J, Zhao H, McMahon A, Yan S. APE1 assembles biomolecular condensates to promote the ATR-Chk1 DNA damage response in nucleolus. Nucleic Acids Res. 2022;50:10503-10525 pubmed publisher
Howpay Manage S, Fleming A, Chen H, Burrows C. Cysteine Oxidation to Sulfenic Acid in APE1 Aids G-Quadruplex Binding While Compromising DNA Repair. ACS Chem Biol. 2022;17:2583-2594 pubmed publisher
Schuermann D, Scheidegger S, Weber A, Bjørås M, Leumann C, Schär P. 3CAPS - a structural AP-site analogue as a tool to investigate DNA base excision repair. Nucleic Acids Res. 2016;44:2187-98 pubmed publisher
product information
Catalog Number :
70757
Product Name :
pET28HIS-hAPE1
article :
doi10.1093/nar/gkv1520
id16461
pubmed_id26733580
bacterial resistance :
Kanamycin
cloning :
backbonepET28c(+)
backbone_mutationmodified MCS including C-terminal thrombin cleavage site and 6xHIS tag
backbone_originNovagen
backbone_size5367
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
bacterial expression of human APE1
origin :
37
pi :
alt_names
APE1
HAP1
REF-1
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5NcoI
promoterT7/lac operon
sequencing_primer_3T7 terminal
sequencing_primer_5pBRrevBam
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAPE, APE1, APEN, APEX, APX, HAP1, REF1
geneAPEX1
id328
genbank_ids
NM_080649.2
mutation
nameAPEX1
shRNA_sequence
size960
species
9606
Homo sapiens
tags
locationC terminal on backbone
tag6-HIS
resistance markers :
1922
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA