This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
R777-E015 Hs.BRAF
catalog :
70299
citations: 2
Reference
Cuevas Navarro A, Wagner M, Van R, Swain M, Mo S, Columbus J, et al. RAS-dependent RAF-MAPK hyperactivation by pathogenic RIT1 is a therapeutic target in Noonan syndrome-associated cardiac hypertrophy. Sci Adv. 2023;9:eadf4766 pubmed publisher
Woolston A, Khan K, Spain G, Barber L, Griffiths B, Gonzalez Exposito R, et al. Genomic and Transcriptomic Determinants of Therapy Resistance and Immune Landscape Evolution during Anti-EGFR Treatment in Colorectal Cancer. Cancer Cell. 2019;36:35-50.e9 pubmed publisher
product information
Catalog Number :
70299
Product Name :
R777-E015 Hs.BRAF
article :
doi
id15959
pubmed_id
bacterial resistance :
Spectinomycin
cloning :
backbonepDonor-255
backbone_mutation
backbone_origin
backbone_size2693
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Gateway Entry Clone
growth strain :
Gateway ORF clone of human BRAF [NM_004333.4] with stop codon (for native or N-terminal fusions)
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3attLR 23-mer (5'-TAACATCAGAGATTTTGAGACAC)
sequencing_primer_5M13F 23-mer (5'-CCCAGTCACGACGTTGTAAAACG)
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesB-RAF1, B-raf, BRAF-1, BRAF1, NS7, RAFB1
geneBRAF
id673
genbank_ids
NM_004333.4
mutation
nameBRAF
shRNA_sequence
size2301
species
9606
Homo sapiens
tags
resistance markers :
139
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA