pLV-tetO-Tfap2a | Addgene 70274 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLV-tetO-Tfap2a

catalog :

70274

citations: 1

Reference
Kubaczka C, Senner C, Cierlitza M, AraÃºzo Bravo M, Kuckenberg P, Peitz M, et al. Direct Induction of Trophoblast Stem Cells from Murine Fibroblasts. Cell Stem Cell. 2015;17:557-68 pubmed publisher

product information

Catalog Number :

70274

Product Name :

pLV-tetO-Tfap2a

article :

doi	10.1016/j.stem.2015.08.005
id	15954
pubmed_id	26412560

bacterial resistance :

Ampicillin

cloning :

backbone

pLV-tetO

backbone_mutation

backbone_origin

Hochedlinger Lab (Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.)

backbone_size

8370

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

The cDNA sequence of murine Tfap2a (coding region and partially 5' and 3' UTR) was inserted into the pLV-tetO vector via the EcoRI site. The pLV-tetO vector backbone was obtained through EcoRI mediated excision of Oct4 cDNA from pLV-tetO-Oct4 (Stadtfeld et al., 2008, addgene Plasmid #19766), kindly provided by K. Hochedlinger (Harvard University).

growth strain :

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Tfap2a

origin :

pi :

alt_names

transcription factor AP-2, alpha

Ap2, Ap-2 (a), AP2alpha, AP-2 alpha, Ap2tf, Tcfap2a

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	EcoRI
promoter	tetO
sequencing_primer_3	WPRE-R: CATAGCGTAAAAGGAGCAACA
sequencing_primer_5	CMV-Promoter F: ACGCCATCCACGCTGTTTTGACCT
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AP-2, AP2alpha, Ap-2 (a), Ap2, Ap2tf, Tcfap2a
gene	Tfap2a
id	21418

genbank_ids

NM_001301674

mutation

name

Tfap2a

shRNA_sequence

size

1900

species

10090

Mus musculus