pNUT N6His K206E/K534A hTFNG | Addgene 70133 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pNUT N6His K206E/K534A hTFNG

catalog :

70133

citations: 1

Reference
Halbrooks P, He Q, Briggs S, Everse S, Smith V, MacGillivray R, et al. Investigation of the mechanism of iron release from the C-lobe of human serum transferrin: mutational analysis of the role of a pH sensitive triad. Biochemistry. 2003;42:3701-7 pubmed

product information

Catalog Number :

70133

Product Name :

pNUT N6His K206E/K534A hTFNG

article :

doi	10.1021/bi027071q
id	15904
pubmed_id	12667060

bacterial resistance :

Ampicillin

cloning :

backbone

pNUT

backbone_mutation

backbone_origin

backbone_size

7400

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

This plasmid to express this double mutant of hTF was prepared in the laboratory of Dr. Ross T.A. MacGillivray using funding from my NIH grant. The plasmid was sent to Vermont for transfection, expression. purification and characterization of the resulting protein

growth strain :

Expresses N-His tagged nonglycosylated human serum transferrin unable to release iron from the N-lobe or the C-lobe

origin :

pi :

alt_names

Mutant hTF double locked

cloning

clone_method	Restriction Enzyme
cloning_site_3	SmaI
cloning_site_5	SmaI
promoter	SV40
sequencing_primer_3	GTGGCAACTTCCAGGGCC
sequencing_primer_5	CCTTTGCGCCCGGACTCG
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	HEL-S-71p, PRO1557, PRO2086, TFQTL1
gene	TF
id	7018

genbank_ids

AF28814

mutation

changed Lys206 to a glutamic acid and Lys534 to an alanine,Asn413Asp and Asn611Asp

name

N6His K206e/K534A hTFNG

shRNA_sequence

size

2136

species

9606

Homo sapiens