This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pNUT N6His K206E hTFNG
catalog :
70130
citations: 1
Reference
He Q, Mason A, Tam B, MacGillivray R, Woodworth R. Dual role of Lys206-Lys296 interaction in human transferrin N-lobe: iron-release trigger and anion-binding site. Biochemistry. 1999;38:9704-11 pubmed
product information
Catalog Number :
70130
Product Name :
pNUT N6His K206E hTFNG
article :
doi10.1021/bi990134t
id15902
pubmed_id10423249
bacterial resistance :
Ampicillin
cloning :
backbonepNUT
backbone_mutation
backbone_origin
backbone_size7400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The MacGillivray lab constructed the plasmid with funds from my NIH grant . They sent it to my lab for transfection of BHK cells, expression, purification and characterization of the inserted mutant
growth strain :
Expresses N-His tagged nonglycosylated human serum transferrin unable to release iron from the N-lobe
origin :
37
pi :
alt_names
K206E or N-locked hTF
cloning
clone_methodRestriction Enzyme
cloning_site_3SmaI
cloning_site_5SmaI
promoterSV40
sequencing_primer_3GTGGCAACTTCCAGGGCC
sequencing_primer_5CCTTTGCGCCCGGACTCG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHEL-S-71p, PRO1557, PRO2086, TFQTL1
geneTF
id7018
genbank_ids
AF28814
mutationchanged Lys206 to a glutamic acid, Asn413Asp, Asn611Asp
namemutated human serum transferrin
shRNA_sequence
size2136
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagN-terminal signal peptide, 4 aa link, 6 His, Factor Xa cleavage site
plasmid copy :
The laboratory of Dr. Ross T.A. MacGillivray at UBC in Vancouver Canada
resistance markers :
2442
2441
tags :
Unknown
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA