pLenti-Synapsin-pHoenix-WPRE | Addgene 70111 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLenti-Synapsin-pHoenix-WPRE

catalog :

70111

citations: 2

Reference
Kim N, Wen Z, Liu J, Zhou Y, Guo Z, Xu C, et al. Pharmacological rescue in patient iPSC and mouse models with a rare DISC1 mutation. Nat Commun. 2021;12:1398 pubmed publisher
Rost B, Schneider F, Grauel M, Wozny C, Bentz C, Blessing A, et al. Optogenetic acidification of synaptic vesicles and lysosomes. Nat Neurosci. 2015;18:1845-1852 pubmed publisher

product information

Catalog Number :

70111

Product Name :

pLenti-Synapsin-pHoenix-WPRE

article :

doi	10.1038/nn.4161
id	16184
pubmed_id	26551543

bacterial resistance :

Ampicillin

cloning :

backbone

FUGW

backbone_mutation

backbone_origin

backbone_size

8400

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Lentiviral

growth strain :

lentiviral expression of pHoenix (Synaptophysin-pHluorin-Arch3-mKate2) driven by human Synapsin I promoter for optogenetic acidification of synaptic vesicles

growth temp :

Researchers should avoid repetitive freeze-thaw cycles when handling the DNA, as we found that this can lead to DNA degradation. Recommended growth strains are XL10gold and Stbl3.

inserts :

XL10-gold

origin :

pi :

alt_names

Synaphtophysin-pHluorin-Arch3-mKate2-betaHK

cloning

clone_method	Restriction Enzyme
cloning_site_3	PacI
cloning_site_5	NheI
promoter	Synapsin
sequencing_primer_3	AGGAGCAACATAGTTAAGAATACC
sequencing_primer_5	CGCTGCCTCAGTCTGCGGTGG
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Syp1
gene	Syp
id	24804

genbank_ids

24804;

KT880224

mutation

name

pHoenix

shRNA_sequence

size

3492

species

10116

Rattus norvegicus

32644

Other

Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria