pLX302_FOXA1-V5 | Addgene 70090 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLX302_FOXA1-V5

catalog :

70090

citations: 3

Reference
Chen Y, Han L, Dufour C, Alfonso A, Giguere V. Canonical and nuclear mTOR specify distinct transcriptional programs in androgen-dependent prostate cancer cells. Mol Cancer Res. 2023;: pubmed publisher
Ferguson L, Gatchalian J, McDermott M, Nakamura M, Chambers K, Rajbhandari N, et al. Smarcd3 is an epigenetic modulator of the metabolic landscape in pancreatic ductal adenocarcinoma. Nat Commun. 2023;14:292 pubmed publisher
Pomerantz M, Li F, Takeda D, Lenci R, Chonkar A, Chabot M, et al. The androgen receptor cistrome is extensively reprogrammed in human prostate tumorigenesis. Nat Genet. 2015;47:1346-51 pubmed publisher

product information

Catalog Number :

70090

Product Name :

pLX302_FOXA1-V5

article :

doi	10.1038/ng.3419
id	15880
pubmed_id	26457646

bacterial resistance :

Ampicillin

cloning :

backbone

pLX_TRC302

backbone_mutation

backbone_origin

backbone_size

9573

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

Please Note- Some scientists have observed an incorrect digest pattern using NotI. Addgene has verified by sequencing that a NotI site is present within the FoxA1 insert.

growth strain :

Expression of human FOXA1 with C-terminal V5 tag, puromycin selection marker, 3rd generation lentiviral vector

origin :

pi :

alt_names

HNF3A

TCF3A

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter	CMV
sequencing_primer_3	pLX_reverse
sequencing_primer_5	pLX_forward
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	HNF3A, TCF3A
gene	FOXA1
id	3169

genbank_ids

NM_004496.3

mutation

name

FOXA1

shRNA_sequence

size

1400

species

9606

Homo sapiens