This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pNUT N6His Y95F/Y188F/Y426F/Y517F hTFNG hTFNG
catalog :
70085
citations: 1
Reference
Mason A, Halbrooks P, Larouche J, Briggs S, Moffett M, Ramsey J, et al. Expression, purification, and characterization of authentic monoferric and apo-human serum transferrins. Protein Expr Purif. 2004;36:318-26 pubmed
product information
Catalog Number :
70085
Product Name :
pNUT N6His Y95F/Y188F/Y426F/Y517F hTFNG hTFNG
article :
doi10.1016/j.pep.2004.04.013
id15745
pubmed_id15249056
bacterial resistance :
Ampicillin
cloning :
backbonepNUT
backbone_mutation
backbone_origin
backbone_size7400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in either the N-lobe or the C-lobeN-lobe
origin :
37
pi :
alt_names
Y95F/Y188F/Y426F/Y517F hTF NG
Authentic apo TF
cloning
clone_methodRestriction Enzyme
cloning_site_3SmaI
cloning_site_5SmaI
promoterSV40
sequencing_primer_3GTGGCAACTTCCAGGGCC
sequencing_primer_5CCTTTGCGCCCGGACTCG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHEL-S-71p, PRO1557, PRO2086, TFQTL1
geneTF
id7018
genbank_ids
AF28814
PRO1557, PRO2086, TFQTL1
mutationAsn413 Asp, Asn611Asp, Tyr95Phe, Tyr188Phe, Ty426Phe, Tyr517Phe
namemutated human serum transferrin
shRNA_sequence
size2136
species
9606
Homo sapiens
tags
locationN terminal on insert
tagN-terminal signal peptide, 4 aa link, 6 His, Factor Xa cleavage site
locationN terminal on insert
tagHexa His tag
plasmid copy :
Dr Ross T.A. MacGillivray UBC Vancouver BC Canada
resistance markers :
2442
2441
tags :
Unknown
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA