pCS-TRE-FHDnd1-Y102A-Ubc-rtTA-I2G | Addgene 70075 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCS-TRE-FHDnd1-Y102A-Ubc-rtTA-I2G

catalog :

70075

citations: 1

Reference
Yamaji M, Jishage M, Meyer C, Suryawanshi H, Der E, Yamaji M, et al. DND1 maintains germline stem cells via recruitment of the CCR4-NOT complex to target mRNAs. Nature. 2017;543:568-572 pubmed publisher

product information

Catalog Number :

70075

Product Name :

pCS-TRE-FHDnd1-Y102A-Ubc-rtTA-I2G

article :

doi	10.1038/nature21690
id	28192353
pubmed_id	28297718

bacterial resistance :

Ampicillin

cloning :

backbone

CS-TRE-mOKS-PRE-Ubc-rTTA-I2G

backbone_mutation

backbone_origin

backbone_size

11522

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

RNP1 of RRM domain is mutated (Y102A). Silent mutations are introduced to escape from RNAi using pLKO.1-shDnd1-No. 1,6, and 8 deposited in Addgene (plasmids #70058, 70067, and 70069).

growth strain :

2nd gen lentiviral vector. tet/dox controllable expression of FLAG-HA-tagged murine Dnd1 (RRM mutant) in mammalian cells

origin :

pi :

musculustags

location	N terminal on backbone
tag	FLAG

location	N terminal on backbone
tag	HA

8nameDnd1d>shRNA_sequenceC
CGGGAAGCAGGTACTATGGTTAAGCTCGAGCTTAACCAT
AGTACCTGCTTCTTTTTGsiz
e1203species

10090

Mus

alt_names

dead end homolog 1

RBMS4

DND microRNA-mediated repression inhibitor 1

cloning

clone_method	Restriction Enzyme
cloning_site_3	NheI
cloning_site_5	EcoRI
promoter	Tet responsible promoter
sequencing_primer_3
sequencing_primer_5	LNCX
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	RBMS4, Ter
gene	Dnd1
id	213236

genbank_ids

213236

mutation

Y102A. Silent mutations are introduced to escape from RNAi using pLKO.1-shDnd1-No1, 6, and

resistance markers :

536

tags :

High Copy