This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLVE-FH-Dnd1-IN
catalog :
70072
citations: 1
Reference
Yamaji M, Jishage M, Meyer C, Suryawanshi H, Der E, Yamaji M, et al. DND1 maintains germline stem cells via recruitment of the CCR4-NOT complex to target mRNAs. Nature. 2017;543:568-572 pubmed publisher
product information
Catalog Number :
70072
Product Name :
pLVE-FH-Dnd1-IN
article :
doi10.1038/nature21690
id28192353
pubmed_id28297718
bacterial resistance :
Ampicillin
cloning :
backbonepLVE-FH-IN
backbone_mutation
backbone_originTuschl lab, Addgene plasmid #70071
backbone_size10748
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
Cre/Lox
growth strain :
2nd gen lentiviral vector. Cre-removable and tet/dox-controllable expression of FLAG-HA-tagged murine Dnd1 in mammalian cells.
origin :
37
pi :
musculustags
locationN terminal on backbone
tagFLAG
locationN terminal on backbone
tagHA

TergeneDnd1<
/td>id213236
r>genbank_idsr>
213236
>mutation>nameDnd1>shRNA_sequenceCCGGGAAGCAGGTAC
TATGGTTAAGCTCGAGCTTAACCATAGTACCTGCTTCTT
TTTGsize1065
speciese>>
10090
Mus
alt_names
dead end homolog 1
RBMS4
DND microRNA-mediated repression inhibitor 1
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterEF1alpha
sequencing_primer_3
sequencing_primer_5EF1a-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesRBMS4,
resistance markers :
536
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA