This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAAV:cTNT::Luciferase
catalog :
69915
citations: 6
Reference
Nishiyama T, Zhang Y, Cui M, Li H, Sanchez Ortiz E, McAnally J, et al. Precise genomic editing of pathogenic mutations in RBM20 rescues dilated cardiomyopathy. Sci Transl Med. 2022;14:eade1633 pubmed publisher
Lu F, Ma Q, Xie W, Liou C, Zhang D, Sweat M, et al. CMYA5 establishes cardiac dyad architecture and positioning. Nat Commun. 2022;13:2185 pubmed publisher
Scerra G, De Pasquale V, Pavone L, Caporaso M, Mayer A, Renna M, et al. Early onset effects of single substrate accumulation recapitulate major features of LSD in patient-derived lysosomes. iScience. 2021;24:102707 pubmed publisher
Makarewich C, Bezprozvannaya S, Gibson A, Bassel Duby R, Olson E. Gene Therapy With the DWORF Micropeptide Attenuates Cardiomyopathy in Mice. Circ Res. 2020;127:1340-1342 pubmed publisher
Guo Q, Zhang Y, Zhang S, Jin J, Pang S, Wu X, et al. Genome-wide translational reprogramming of genes important for myocyte functions in overload-induced heart failure. Biochim Biophys Acta Mol Basis Dis. 2020;1866:165649 pubmed publisher
Lin Z, von Gise A, Zhou P, Gu F, Ma Q, Jiang J, et al. Cardiac-specific YAP activation improves cardiac function and survival in an experimental murine MI model. Circ Res. 2014;115:354-63 pubmed publisher
product information
Catalog Number :
69915
Product Name :
pAAV:cTNT::Luciferase
article :
doi10.1161/CIRCRESAHA.115.303632
id15821
pubmed_id24833660
bacterial resistance :
Ampicillin
cloning :
backboneAAV2/9.cTnT.PI.EGFP.RBG
backbone_mutation
backbone_originPenn Vector Core
backbone_size4405
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
AAV
growth notes :
This plasmid contains chicken cardiac tromonin T promoter. When packaged into AAV9 virus using the AAV 2/9 Rep/Cap plasmid, the AAV9:cTNT.Luciferease virus will easily transduce the neonatal mouse. This virus does not work in vitro cultured cardiomyocytes.
growth strain :
Used to package AAV9:cTNT::luciferase. Specifically transduce cardiomyocytes.
growth temp :
The plasmid will easily get mutated if bacterial cultured too long. Using SmaI to do a diagnose digestion is recommended.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5NheI
promoterChicken cardiac troponin T
sequencing_primer_3CCAGAAGTCAGATGCTCAAG
sequencing_primer_5CCAATAGAAACTGGGCTTGTC
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
Nuleotide> U47295
mutation
nameFirefly luciferase
shRNA_sequence
size1653
species
32644
Other
Firefly
tags
plasmid copy :
The luciferase gene was PCR amplified from pGL basic vector (Promega) .
resistance markers :
964
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA