This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Beta1-GFP in pHcgreen
catalog :
69804
citations: 5
Reference
Alfonzo Méndez M, Sochacki K, Strub M, Taraska J. Dual clathrin and integrin signaling systems regulate growth factor receptor activation. Nat Commun. 2022;13:905 pubmed publisher
Castro Córdova P, Mora Uribe P, Reyes Ramírez R, Cofre Araneda G, Orozco Aguilar J, Brito Silva C, et al. Entry of spores into intestinal epithelial cells contributes to recurrence of Clostridioides difficile infection. Nat Commun. 2021;12:1140 pubmed publisher
Shen M, Jiang Y, Wei Y, Ell B, Sheng X, Esposito M, et al. Tinagl1 Suppresses Triple-Negative Breast Cancer Progression and Metastasis by Simultaneously Inhibiting Integrin/FAK and EGFR Signaling. Cancer Cell. 2019;35:64-80.e7 pubmed publisher
Kim S, Yooun J, Kim D, Lee E, Kwon T, Kim S, et al. A novel anti-cancer agent, FPDHP, induces anoikis in various human cancer cells through activation of calpain, and downregulation of anoikis-related molecules. J Cell Biochem. 2018;119:5620-5631 pubmed publisher
Parsons M, Messent A, Humphries J, Deakin N, Humphries M. Quantification of integrin receptor agonism by fluorescence lifetime imaging. J Cell Sci. 2008;121:265-71 pubmed publisher
product information
Catalog Number :
69804
Product Name :
Beta1-GFP in pHcgreen
article :
doi10.1242/jcs.018440
id15655
pubmed_id18216331
bacterial resistance :
Kanamycin
cloning :
backbonepHcRed1-N1/1
backbone_mutationafter cloning Beta1 into pHcRed the RFp fluorophore sequence was excised usin Age1 and Not1, and the GFP sequence from pEGFPN1 cloned into the pHcRed backbone using the same restriction sites
backbone_originClontech
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses Beta1-GFP integrin for normal cell transfection
origin :
37
pi :
alt_names
ITGB1
cloning
clone_methodRestriction Enzyme
cloning_site_3Age1
cloning_site_5Kpn1
promoterCMV
sequencing_primer_3SV40A-R
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCD29, FNRB, GPIIA, MDF2, MSK12, VLA-BETA, VLAB
geneITGB1
id3688
genbank_ids
NM_002211.3
mutation
nameBeta1A integrin
shRNA_sequence
size3700
species
9606
Homo sapiens
tags
location
tagGFP
resistance markers :
2559
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA