This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3.2/DEST/hTfR-HA
catalog :
69610
citations: 4
Reference
Gardner J, Leidal A, Nguyen T, Debnath J. LC3-dependent EV loading and secretion (LDELS) promotes TFRC (transferrin receptor) secretion via extracellular vesicles. Autophagy. 2022;:1-11 pubmed publisher
Bishayee K, Habib K, Sadra A, Huh S. Targeting the Difficult-to-Drug CD71 and MYCN with Gambogic Acid and Vorinostat in a Class of Neuroblastomas. Cell Physiol Biochem. 2019;53:258-280 pubmed publisher
Ghosh E, Srivastava A, Baidya M, Kumari P, Dwivedi H, Nidhi K, et al. A synthetic intrabody-based selective and generic inhibitor of GPCR endocytosis. Nat Nanotechnol. 2017;12:1190-1198 pubmed publisher
Smyth J, Vogan J, Buch P, Zhang S, Fong T, Hong T, et al. Actin cytoskeleton rest stops regulate anterograde traffic of connexin 43 vesicles to the plasma membrane. Circ Res. 2012;110:978-89 pubmed publisher
product information
Catalog Number :
69610
Product Name :
pcDNA3.2/DEST/hTfR-HA
article :
doi10.1161/CIRCRESAHA.111.257964
id5965
pubmed_id22328533
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.2-HA/Dest (converted from pcDNA3.2-V5/Dest)
backbone_mutationpcDNA3.2-V5/Dest was converted to pcDNA3.2-HA/Dest by first introducing unique NotI and XhoI sites by sire-directed mutagenesis, followed by ligation of annealed HA-stop peptide.
backbone_originInvitrogen
backbone_size7699
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
human transferrin receptor with an HA tag fused at the C-terminus
origin :
37
pi :
alt_names
TfR
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3TfR internal primer (5' CGCTGCCAGCTTTACTGGAG 3')
sequencing_primer_5T7 forward primer
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCD71, IMD46, T9, TFR, TFR1, TR, TRFR, p90
geneTFRC
id7037
genbank_ids
BC001188
mutation
namehuman transferrin receptor
shRNA_sequence
size2283
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagHA
resistance markers :
877
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA