p3A9cbetaTCR | Addgene 69600 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

p3A9cbetaTCR

catalog :

69600

citations: 1

Reference
Barnden M, Allison J, Heath W, Carbone F. Defective TCR expression in transgenic mice constructed using cDNA-based alpha- and beta-chain genes under the control of heterologous regulatory elements. Immunol Cell Biol. 1998;76:34-40 pubmed

product information

Catalog Number :

69600

Product Name :

p3A9cbetaTCR

article :

doi	10.1046/j.1440-1711.1998.00709.x
id	15739
pubmed_id	9553774

bacterial resistance :

Ampicillin

cloning :

backbone

pBluescript KS

backbone_mutation

See comments

backbone_origin

Invitrogen

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

A 1.0 kb Sma-I/Not-I fragment containing the TCR b-chain enhancer element was excised from pKS913.Cb18.31 and cloned into the pKS Bluescript plasmid (Stratagene), which had been modified to remove the Kpn-I and Cla-I restriction sites from the polylinker resulting in the pKS.bEnh plasmid. A Sal-I fragment, containing the B3 TCR b-chain enhancer, promoter and coding regions, was isolated from pKS913.Cb18.31 and cloned into the Sal-I site of the pKS.bEnh plasmid to give the construct pKS913.Cb18.31(Kpn-I)/Cla-I)). A 4.2 kb Eco-I/Cla-I fragment comprising the 5 region and leader sequence from the hybridoma 2B4 was isolated from the p3A9 b shuttle vector. This fragment was cloned into the pGEM-T plasmid (Promega) resulting in the pG3A9 plasmid. A 4.2 kb Apa-I/Cla-I fragment, containing the 2B4 5 region and leader sequence, was isolated from the pG3A9 plasmid. This fragment was cloned into pKS319.Cb18.31(Kpn-I)/Cla-I)) after removal of the B3 TCR b-chain promoter and the TCRBVDJ coding regions by a combined Apa-I/Cla-I restriction enzyme digest, resulting in the p3A9cbTCR plasmid.

growth strain :

TCR beta chain transgene expression plasmid which has a unique ClaI site for the insertion of a TCR beta chain VDJ exon

origin :

resistance markers :

2549

tags :

Unknown