This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRRLHygro-pEF1a-p53ashL344A-mKate2-splitmVenusC
catalog :
69585
citations: 1
Reference
Gaglia G, Lahav G. Constant rate of p53 tetramerization in response to DNA damage controls the p53 response. Mol Syst Biol. 2014;10:753 pubmed publisher
product information
Catalog Number :
69585
Product Name :
pRRLHygro-pEF1a-p53ashL344A-mKate2-splitmVenusC
article :
doi
id15736
pubmed_id25344068
bacterial resistance :
Ampicillin
cloning :
backbonepRRL
backbone_mutationhygromycin resistance
backbone_origin
backbone_size7765
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
The silent mutations prevent targeting by shRNA p53 published in Brummelkamp et al Science. 2002;296:550 3. This is tagged with the C-terminus of a split mVenus. The L344A mutation prevents tetramerization, but not dimerization of p53.
growth strain :
Expresses mutated p53-L344A tagged with mKate2 and split C-term mVenus
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterEF1alpha
sequencing_primer_3
sequencing_primer_5CAAGTTTGTACAAAAAAGCAGGCT
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBCC7, BMFS5, LFS1, P53, TRP53
geneTP53
id7157
genbank_ids
mutationp53 L344A mutation that prevents tetramerization and 7 silent mutations in p53 DNA to prevent targeting by shRNA at positions C777T, T778A, C779G, A781T, G782C, T786A, T789C
namep53-L344A
shRNA_sequence
size1179
species
9606
Homo sapiens
tags
locationC terminal on insert
tagmKate2
locationC terminal on insert
tagsplit mVenus - C term
resistance markers :
1977
tags :
Low Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA