This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pRRLNeo-pEF1a-p53ash-mCerulean
catalog :
69579
citations: 1
Reference
Gaglia G, Guan Y, Shah J, Lahav G. Activation and control of p53 tetramerization in individual living cells. Proc Natl Acad Sci U S A. 2013;110:15497-501 pubmed publisher
product information
Catalog Number :
69579
Product Name :
pRRLNeo-pEF1a-p53ash-mCerulean
article :
doi10.1073/pnas.1311126110
id15735
pubmed_id24006363
bacterial resistance :
Ampicillin
cloning :
backbonepRRL
backbone_mutationneomycin resistance
backbone_origin
backbone_size7429
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
The silent mutations prevent targeting by shRNA p53 published in Brummelkamp et al Science. 2002;296:550 3.
growth strain :
Expresses p53 tagged with mCerulean
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterEF1alpha
sequencing_primer_3
sequencing_primer_5CAAGTTTGTACAAAAAAGCAGGCT
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBCC7, BMFS5, LFS1, P53, TRP53
geneTP53
id7157
genbank_ids
mutation7 silent mutations in p53 DNA to prevent targeting by shRNA at positions C777T, T778A, C779G, A781T, G782C, T786A, T789C
namep53
shRNA_sequence
size1179
species
9606
Homo sapiens
tags
locationC terminal on insert
tagmCerulean
resistance markers :
1977
tags :
Low Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA