This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pattB-aubergine-ADH-gf
catalog :
69448
citations: 1
Reference
Tang X, Li T, Liu S, Wisniewski J, Zheng Q, Rong Y, et al. Kinetic principles underlying pioneer function of GAGA transcription factor in live cells. Nat Struct Mol Biol. 2022;29:665-676 pubmed publisher
product information
Catalog Number :
69448
Product Name :
pattB-aubergine-ADH-gf
article :
doi
id15682
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepattB
backbone_mutation
backbone_origin
backbone_size7411
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth strain :
genomic fragment of Drosophila aubergine was cloned from w1 background and inserted into pattB plasmid; mutagenesis PCR was used to change the catalytic residue DDH to ADH to express cat-dead Aub
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3Not I
cloning_site_5Not I
promoteraubergine
sequencing_primer_3TGC ATA AGG TTG CCC TAC CA
sequencing_primer_5TCC CTT AGG AGC CTC AGC AT
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDmel_CG6137, AUB, AUBERGINE, Aub, CG6137, Dmel CG6137, ms(2)32D, sti, sting
geneaub
id34524
genbank_ids
mutationchr2L:10,998,626( )T>G
nameaubergine
shRNA_sequence
size9821
species
7227
Drosophila melanogaster
tags
location
tagNA
resistance markers :
1193
tags :
High Copy
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA