This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGEX-4T1-RhoA G17A
catalog :
69357
citations: 3
Reference
Sajib M, Zahra F, Akwii R, Mikelis C. Identification of Rho GEF and RhoA Activation by Pull-Down Assays. Methods Mol Biol. 2021;2193:97-109 pubmed publisher
Jiu Y, Peranen J, Schaible N, Cheng F, Eriksson J, Krishnan R, et al. Vimentin intermediate filaments control actin stress fiber assembly through GEF-H1 and RhoA. J Cell Sci. 2017;130:892-902 pubmed publisher
Garcia Mata R, Wennerberg K, Arthur W, Noren N, Ellerbroek S, Burridge K. Analysis of activated GAPs and GEFs in cell lysates. Methods Enzymol. 2006;406:425-37 pubmed
product information
Catalog Number :
69357
Product Name :
pGEX-4T1-RhoA G17A
article :
doi10.1016/S0076-6879(06)06031-9
id5370
pubmed_id16472675
bacterial resistance :
Ampicillin
cloning :
backbonepGEX-4T1
backbone_mutation
backbone_originGE
backbone_size4900
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
RhoA has a mutation, G17A, which makes it nucleotide free when expressed in bacteria, which can be used GEF pulldowns.
growth strain :
expression of nucleotide free RhoA in bacterial cells
origin :
37
pi :
alt_names
ARH12
ARHA
RHO12
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5EcoRI
promotertac
sequencing_primer_3pGEX3
sequencing_primer_5pGEX5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesARH12, ARHA, EDFAOB, RHO12, RHOH12
geneRHOA
id387
genbank_ids
387
mutationG17A
nameRhoA
shRNA_sequence
size579
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagGST
plasmid copy :
Keith Burridge Lab, UNC Chapel Hill
resistance markers :
2538
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA