This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGEX-4T1-Rac1 G15A
catalog :
69355
citations: 1
Reference
Garcia Mata R, Wennerberg K, Arthur W, Noren N, Ellerbroek S, Burridge K. Analysis of activated GAPs and GEFs in cell lysates. Methods Enzymol. 2006;406:425-37 pubmed
product information
Catalog Number :
69355
Product Name :
pGEX-4T1-Rac1 G15A
article :
doi10.1016/S0076-6879(06)06031-9
id5370
pubmed_id16472675
bacterial resistance :
Ampicillin
cloning :
backbonepGEX-4T1
backbone_mutation
backbone_originGE
backbone_size4900
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Rac1 has a mutation, G15A, which makes it nucleotide free when expressed in bacteria, which can be used GEF pulldowns.
growth strain :
expression of nucleotide free Rac1 in bacterial cells
origin :
37
pi :
alt_names
MIG5
TC-25
p21-Rac1
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5EcoRI
promotertac
sequencing_primer_3pGEX3
sequencing_primer_5pGEX5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesMIG5, MRD48, Rac-1, TC-25, p21-Rac1
geneRAC1
id5879
genbank_ids
5879
mutationG15A
nameRac1
shRNA_sequence
size576
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagGST
plasmid copy :
Keith Burridge Lab, UNC Chapel Hill
resistance markers :
2538
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA