This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSR13
catalog :
69155
citations: 1
Reference
Ruijtenberg S, van den Heuvel S. G1/S Inhibitors and the SWI/SNF Complex Control Cell-Cycle Exit during Muscle Differentiation. Cell. 2015;162:300-313 pubmed publisher
product information
Catalog Number :
69155
Product Name :
pSR13
article :
doi10.1016/j.cell.2015.06.013
id15643
pubmed_id26144318
bacterial resistance :
Ampicillin
cloning :
backbonepCFJ350
backbone_mutation
backbone_originErik Jorgensen
backbone_size7517
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Worm Expression
Cre/Lox
Other
MosSCI
growth notes :
discrepancies between QC and full sequence should not have functional consequences
growth strain :
read-outloxN mCherry to GFP switch, with swsn-1 promoter, gene (partially cDNA) and UTR, for integration on on ttTi5605, Mos Chr II
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterrps-27
sequencing_primer_3N/A
sequencing_primer_5M13 reverse
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutationcodon-optimzed index 1.0
namemCherry
shRNA_sequence
size861
species
6239
Caenorhabditis elegans
tags
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterswsn-1
sequencing_primer_3N/A
sequencing_primer_5N/A
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCELE_Y113G7B.23
geneswsn-1
id180324
genbank_ids
mutationfirst exon plus intron, then cDNA
nameswsn-1
shRNA_sequence
size6058
species
6239
Caenorhabditis elegans
tags
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3N/A
sequencing_primer_5N/A
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutationcodon-optimzed index 1.0, A to T mutation at bp 793 in GFP, GFP still visible but possibly weaker
nameeGFP
shRNA_sequence
size869
species
6239
Caenorhabditis elegans
tags
resistance markers :
544
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA