pTM1_VP30_ZEBOV | Addgene 69119 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pTM1_VP30_ZEBOV

catalog :

69119

citations: 3

Reference
Levine C, Mire C, Geisbert T. Comparison of Zaire and Bundibugyo Ebolavirus Polymerase Complexes and Susceptibility to Antivirals through a Newly Developed Bundibugyo Minigenome System. J Virol. 2021;95:e0064321 pubmed publisher
Manhart W, Pacheco J, Hume A, Cressey T, Deflubé L, Mühlberger E. A Chimeric Lloviu Virus Minigenome System Reveals that the Bat-Derived Filovirus Replicates More Similarly to Ebolaviruses than Marburgviruses. Cell Rep. 2018;24:2573-2580.e4 pubmed publisher
Trunschke M, Conrad D, Enterlein S, Olejnik J, Brauburger K, M hlberger E. The L-VP35 and L-L interaction domains reside in the amino terminus of the Ebola virus L protein and are potential targets for antivirals. Virology. 2013;441:135-45 pubmed publisher

product information

Catalog Number :

69119

Product Name :

pTM1_VP30_ZEBOV

article :

doi	10.1016/j.virol.2013.03.013
id	14575
pubmed_id	23582637

bacterial resistance :

Ampicillin

cloning :

backbone

pTM1

backbone_mutation

backbone_origin

Bernhard Moss, National Institutes of Health

backbone_size

5357

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Other

growth strain :

Encodes for Ebola Virus Zaire VP30 under control of a T7 RNA polymerase promoter

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	PstI
cloning_site_5	BamHI
promoter	T7
sequencing_primer_3	GCC AAC TCA GCT TCC TTT CGG
sequencing_primer_5	ATT GTA TGG GAT CTG ATC TGG
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ZEBOVgp5
gene	VP30
id	911826

genbank_ids

mutation

name

VP30 ZEBOV

shRNA_sequence

size

866

species