p11-LacY-wtx1 | Addgene 69056 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

p11-LacY-wtx1

catalog :

69056

citations: 7

Reference
Herring Nicholas A, Dimig H, Roesing M, Josephs E. Selection of extended CRISPR RNAs with enhanced targeting and specificity. Commun Biol. 2024;7:86 pubmed publisher
Schmidheini L, Mathis N, Marquart K, Rothgangl T, Kissling L, B xf6 ck D, et al. Continuous directed evolution of a compact CjCas9 variant with broad PAM compatibility. Nat Chem Biol. 2023;: pubmed publisher
Herring Nicholas A, Dimig H, Roesing M, Josephs E. Selection of Extended CRISPR RNAs with Enhanced Targeting and Specificity. bioRxiv. 2023;: pubmed publisher
Ciciani M, Demozzi M, Pedrazzoli E, Visentin E, Pezz xe8 L, Signorini L, et al. Automated identification of sequence-tailored Cas9 proteins using massive metagenomic data. Nat Commun. 2022;13:6474 pubmed publisher
Christie K, Guo J, Silverstein R, Doll R, Mabuchi M, Stutzman H, et al. Precise DNA cleavage using CRISPR-SpRYgests. Nat Biotechnol. 2022;: pubmed publisher
Kleinstiver B, Sousa A, Walton R, Tak Y, Hsu J, Clement K, et al. Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Nat Biotechnol. 2019;37:276-282 pubmed publisher
Chen Z, Zhao H. A highly sensitive selection method for directed evolution of homing endonucleases. Nucleic Acids Res. 2005;33:e154 pubmed

product information

Catalog Number :

69056

Product Name :

p11-LacY-wtx1

article :

doi	10.1093/nar/gni148
id	15623
pubmed_id	16214805

bacterial resistance :

Ampicillin

cloning :

backbone	pBAD18
backbone_mutation
backbone_origin
backbone_size	4600
promoter
sequencing_primer_3
sequencing_primer_5
vector_types

growth notes :

Note: Some users of this plasmid report low DNA yields from DNA preps. Addgene recommends users be aware of this when prepping DNA from this plasmid.

growth strain :

The reporter plasmid p11-LacY-wtx1 encodes the toxin CcdB gene under the inducible BAD promoter and a single copy of the endonuclease cleavage site which is readily extendable to multiple copies.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	SphI
cloning_site_5	NheI
promoter	pBAD
sequencing_primer_3	agtacg gcatgc cgtatg tctaga ttatattccccaaaacatcaggttaat
sequencing_primer_5	catgca gctagc GGAGTG aaacgatgcagttt aaggtttacacctataaaaga
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	pCoo051
gene	ccdB
id	3829332

genbank_ids

mutation

name

ccdB

shRNA_sequence

size

species