sfGFP-Cx43K258stop | Addgene 69025 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

sfGFP-Cx43K258stop

catalog :

69025

citations: 2

Reference
Fonseca J, Aslankoohi E, Ng A, Chevalier M. Analysis of localized cAMP perturbations within a tissue reveal the effects of a local, dynamic gap junction state on ERK signaling. PLoS Comput Biol. 2022;18:e1009873 pubmed publisher
Stout R, Snapp E, Spray D. Connexin Type and Fluorescent Protein Fusion Tag Determine Structural Stability of Gap Junction Plaques. J Biol Chem. 2015;290:23497-514 pubmed publisher

product information

Catalog Number :

69025

Product Name :

sfGFP-Cx43K258stop

article :

doi	10.1074/jbc.M115.659979
id	15872
pubmed_id	26265468

bacterial resistance :

Kanamycin

cloning :

backbone

EGFP-C1

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Note all connexins tested that have a fluorescent protein fused to the connexin amino-terminus do not form functional channels. This plasmid forms gap junction plaque structures but does not produce intercellular coupling.

growth strain :

Expresses rat Cx43 (Gja1 CDS) truncated at amino acid 258 and tagged with sfGFP (V206-version) on the N-terminus. CMV promoter.

origin :

pi :

alt_names

cloning

clone_method	Ligation Independent Cloning
cloning_site_3
cloning_site_5
promoter	CMV
sequencing_primer_3	C1N1-rev: ACCTCTACAAATGTGGTATGGCTGATTATG
sequencing_primer_5	CMV
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Cx43, Cxnk1
gene	Gja1
id	24392

genbank_ids

mutation

non-monomerized sfGFP fused in frame to N-terminus of truncated Cx43delete codons 258-381 of the Cx43 CDS

name

Cx43

shRNA_sequence

size

768

species

10116

Rattus norvegicus