msfGFP-Cx43 | Addgene 69024 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

msfGFP-Cx43

catalog :

69024

citations: 4

Reference
Soto J, Jami Alahmadi Y, Chacon J, Moye S, Diaz Castro B, Wohlschlegel J, et al. Astrocyte-neuron subproteomes and obsessive-compulsive disorder mechanisms. Nature. 2023;616:764-773 pubmed publisher
Fonseca J, Aslankoohi E, Ng A, Chevalier M. Analysis of localized cAMP perturbations within a tissue reveal the effects of a local, dynamic gap junction state on ERK signaling. PLoS Comput Biol. 2022;18:e1009873 pubmed publisher
Murphy Royal C, Johnston A, Boyce A, Diaz Castro B, Institoris A, Peringod G, et al. Stress gates an astrocytic energy reservoir to impair synaptic plasticity. Nat Commun. 2020;11:2014 pubmed publisher
Stout R, Snapp E, Spray D. Connexin Type and Fluorescent Protein Fusion Tag Determine Structural Stability of Gap Junction Plaques. J Biol Chem. 2015;290:23497-514 pubmed publisher

product information

Catalog Number :

69024

Product Name :

msfGFP-Cx43

article :

doi	10.1074/jbc.M115.659979
id	15872
pubmed_id	26265468

bacterial resistance :

Kanamycin

cloning :

backbone

EGFP-N1

backbone_mutation

EGFP was previously swapped for EBFP2

backbone_origin

Clontech

backbone_size

4700

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Note all connexins tested that have a fluorescent protein fused to the connexin amino-terminus do not form functional channels. This plasmid forms gap junction plaque structures but does not produce intercellular coupling.

growth strain :

Expresses rat Cx43 (Gja1 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to msfGFP (FP fused to NT of connexin). CMV promoter.

origin :

pi :

alt_names

Gja1

cloning

clone_method	Ligation Independent Cloning
cloning_site_3
cloning_site_5
promoter	CMV
sequencing_primer_3	C1N1-rev: ACCTCTACAAATGTGGTATGGCTGATTATG
sequencing_primer_5	CMV
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Cx43, Cxnk1
gene	Gja1
id	24392

genbank_ids

mutation

name

Cx43

shRNA_sequence

size

species

10116

Rattus norvegicus