This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Cx43K258stop-msfGFP
catalog :
69023
citations: 1
Reference
Stout R, Snapp E, Spray D. Connexin Type and Fluorescent Protein Fusion Tag Determine Structural Stability of Gap Junction Plaques. J Biol Chem. 2015;290:23497-514 pubmed publisher
product information
Catalog Number :
69023
Product Name :
Cx43K258stop-msfGFP
article :
doi10.1074/jbc.M115.659979
id15872
pubmed_id26265468
bacterial resistance :
Kanamycin
cloning :
backboneEGFP-N1
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Addition of a fluorescent protein tag to the carboxyl-terminus of Cx43 has been shown to eliminate or attenuate interactions with ZO-1 and Occludin in cell culture. Other binding interactions and channel gating properties may be affected.
growth strain :
Expresses rat Cx43 (Gja1 CDS) truncated at amino acid 258 and tagged with msfGFP on the C-terminus. CMV promoter.
origin :
37
pi :
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3C1N1-rev: ACCTCTACAAATGTGGTATGGCTGATTATG
sequencing_primer_5CMV
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCx43, Cxnk1
geneGja1
id24392
genbank_ids
mutationdelete codons 258-381 and fuse in frame to monomeric superfolder GFP
nameCx43
shRNA_sequence
size768
species
10116
Rattus norvegicus
tags
locationC terminal on insert
tagV206K monomerized super folder GFP
resistance markers :
2583
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA