EBFP2-Cx43 | Addgene 69020 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

EBFP2-Cx43

catalog :

69020

citations: 1

Reference
Stout R, Snapp E, Spray D. Connexin Type and Fluorescent Protein Fusion Tag Determine Structural Stability of Gap Junction Plaques. J Biol Chem. 2015;290:23497-514 pubmed publisher

product information

Catalog Number :

69020

Product Name :

EBFP2-Cx43

article :

doi	10.1074/jbc.M115.659979
id	15872
pubmed_id	26265468

bacterial resistance :

Kanamycin

cloning :

backbone	EGFP-C1
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types

growth notes :

Note all connexins tested that have a fluorescent protein fused to the connexin amino-terminus do not form functional channels. This plasmid forms gap junction plaque structures but does not produce intercellular coupling.

growth strain :

Expresses rat Cx43 (Gja1 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.

origin :

pi :

alt_names

cloning

clone_method	Ligation Independent Cloning
cloning_site_3
cloning_site_5
promoter	CMV
sequencing_primer_3	C1N1-rev: ACCTCTACAAATGTGGTATGGCTGATTATG
sequencing_primer_5	CMV
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Cx43, Cxnk1
gene	Gja1
id	24392

genbank_ids

mutation

name

Cx43

shRNA_sequence

size

1146

species

10116

Rattus norvegicus