This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-FLAG-MTOR-S2215Y
catalog :
69013
citations: 7
Reference
Nguyen L, Xu Y, Nair M, Bordey A. The mTOR pathway genes mTOR, Rheb, Depdc5, Pten, and Tsc1 have convergent and divergent impacts on cortical neuron development and function. bioRxiv. 2023;: pubmed publisher
Liu H, Du H, Khabibullin D, Zarei M, Wei K, Freeman G, et al. mTORC1 upregulates B7-H3/CD276 to inhibit antitumor T cells and drive tumor immune evasion. Nat Commun. 2023;14:1214 pubmed publisher
Chen X, Adhikary G, Newland J, Xu W, Ma E, Naselsky W, et al. The transglutaminase 2 cancer cell survival factor maintains mTOR activity to drive an aggressive cancer phenotype. Mol Carcinog. 2023;62:90-100 pubmed publisher
Park H, Kim T, Lee Y, Kim S, Park S, Ju Y, et al. Acquired Resistance to Third-Generation EGFR Tyrosine Kinase Inhibitors in Patients With De Novo EGFRT790M-Mutant NSCLC. J Thorac Oncol. 2021;: pubmed publisher
Gremke N, Polo P, Dort A, Schneikert J, Elmshäuser S, Brehm C, et al. mTOR-mediated cancer drug resistance suppresses autophagy and generates a druggable metabolic vulnerability. Nat Commun. 2020;11:4684 pubmed publisher
Kadoya M, Sasai N. Negative Regulation of mTOR Signaling Restricts Cell Proliferation in the Floor Plate. Front Neurosci. 2019;13:1022 pubmed publisher
Grabiner B, Nardi V, Birsoy K, Possemato R, Shen K, Sinha S, et al. A diverse array of cancer-associated MTOR mutations are hyperactivating and can predict rapamycin sensitivity. Cancer Discov. 2014;4:554-63 pubmed publisher
product information
Catalog Number :
69013
Product Name :
pcDNA3-FLAG-MTOR-S2215Y
article :
doi10.1158/2159-8290.CD-13-0929
id10004
pubmed_id24631838
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_origin
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Activating mutations in MTOR were generated using site-directed mutagenesis on AddGene plasmid 26603 pcDNA3-Flag mTOR wt deposited by Jie Chen lab Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72.
growth strain :
activating MTOR mutation
origin :
37
pi :
alt_names
MTOR (a.k.a. FRAP, FRAP1, FRAP2, RAFT1, RAPT1
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesFRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS
geneMTOR
id2475
genbank_ids
NM_004958
mutationS2215Y
nameMTOR
shRNA_sequence
size7650
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagFLAG
plasmid copy :
AddGene pcDNA3-Flag mTOR wt (Plasmid #26603)
resistance markers :
432
tags :
Low Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA