This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-FLAG-MTOR-F1888L
catalog :
69010
citations: 1
Reference
Grabiner B, Nardi V, Birsoy K, Possemato R, Shen K, Sinha S, et al. A diverse array of cancer-associated MTOR mutations are hyperactivating and can predict rapamycin sensitivity. Cancer Discov. 2014;4:554-63 pubmed publisher
product information
Catalog Number :
69010
Product Name :
pcDNA3-FLAG-MTOR-F1888L
article :
doi10.1158/2159-8290.CD-13-0929
id10004
pubmed_id24631838
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_origin
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Activating mutations in MTOR were generated using site-directed mutagenesis on AddGene plasmid 26603 pcDNA3-Flag mTOR wt deposited by Jie Chen lab Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72.
growth strain :
activating MTOR mutation
origin :
37
pi :
alt_names
MTOR (a.k.a. FRAP, FRAP1, FRAP2, RAFT1, RAPT1
cloning
entrez_gene
aliasesFRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS
geneMTOR
id2475
genbank_ids
NM_004958
mutationchange Phenylalanine 1888 to Leucine
nameMTOR-F1888L
shRNA_sequence
size7650
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagFLAG
plasmid copy :
AddGene pcDNA3-Flag mTOR wt (Plasmid #26603)
resistance markers :
432
tags :
Low Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA