Cx43-msfGFP | Addgene 69007 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

Cx43-msfGFP

catalog :

69007

citations: 1

Reference
Stout R, Snapp E, Spray D. Connexin Type and Fluorescent Protein Fusion Tag Determine Structural Stability of Gap Junction Plaques. J Biol Chem. 2015;290:23497-514 pubmed publisher

product information

Catalog Number :

69007

Product Name :

Cx43-msfGFP

article :

doi	10.1074/jbc.M115.659979
id	15872
pubmed_id	26265468

bacterial resistance :

Kanamycin

cloning :

backbone

EGFP-N1

backbone_mutation

EGFP replaced with sfGFP and the V207 in sfGFP mutagenized to K with PCR cloning to monomerize.

backbone_origin

Clontech

backbone_size

4733

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Addition of a fluorescent protein tag to the carboxyl-terminus of Cx43 has been shown to eliminate or attenuate interactions with ZO-1 and Occludin in cell culture. Other binding interactions and channel gating properties may be affected.

growth strain :

Expresses rat Cx43 (Gja1 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.

origin :

pi :

alt_names

Cx43

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter	cmv
sequencing_primer_3	ACCTCTACAAATGTGGTATGGCTGATTATG
sequencing_primer_5	CMV forward primer
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Cx43, Cxnk1
gene	Gja1
id	24392

genbank_ids

mutation

name

Gja1

shRNA_sequence

size

species

10116

Rattus norvegicus