This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-FLAG-MTOR-L1460P
catalog :
69006
citations: 5
Reference
Liu H, Du H, Khabibullin D, Zarei M, Wei K, Freeman G, et al. mTORC1 upregulates B7-H3/CD276 to inhibit antitumor T cells and drive tumor immune evasion. Nat Commun. 2023;14:1214 pubmed publisher
Zhang S, Lee S, Nie L, Huang Y, Zou G, Jung Y, et al. Lysosomal TMEM9-LAMTOR4-controlled mTOR signaling integrity is required for mammary tumorigenesis. Cancer Commun (Lond). 2022;: pubmed publisher
Gremke N, Polo P, Dort A, Schneikert J, Elmshäuser S, Brehm C, et al. mTOR-mediated cancer drug resistance suppresses autophagy and generates a druggable metabolic vulnerability. Nat Commun. 2020;11:4684 pubmed publisher
Ochocki J, Khare S, Hess M, Ackerman D, Qiu B, Daisak J, et al. Arginase 2 Suppresses Renal Carcinoma Progression via Biosynthetic Cofactor Pyridoxal Phosphate Depletion and Increased Polyamine Toxicity. Cell Metab. 2018;27:1263-1280.e6 pubmed publisher
Grabiner B, Nardi V, Birsoy K, Possemato R, Shen K, Sinha S, et al. A diverse array of cancer-associated MTOR mutations are hyperactivating and can predict rapamycin sensitivity. Cancer Discov. 2014;4:554-63 pubmed publisher
product information
Catalog Number :
69006
Product Name :
pcDNA3-FLAG-MTOR-L1460P
article :
doi10.1158/2159-8290.CD-13-0929
id10004
pubmed_id24631838
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_origin
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Activating mutations in MTOR were generated using site-directed mutagenesis on AddGene plasmid 26603 pcDNA3-Flag mTOR wt deposited by Jie Chen lab Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72.
growth strain :
activating MTOR mutation
origin :
37
pi :
alt_names
MTOR (a.k.a. FRAP, FRAP1, FRAP2, RAFT1, RAPT1
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesFRAP, FRAP1, FRAP2, RAFT1, RAPT1, SKS
geneMTOR
id2475
genbank_ids
mutationchange Leucine 1460 to Proline
nameMTOR-L1460P
shRNA_sequence
size7650
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagFLAG
plasmid copy :
AddGene pcDNA3-Flag mTOR wt (Plasmid #26603)
resistance markers :
432
tags :
Low Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA