This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pE478G-EGFP
catalog :
68848
citations: 2
Reference
Cazzaro S, Fang C, Khan H, Witas R, Kee T, Woo J, et al. Slingshot homolog-1 mediates the secretion of small extracellular vesicles containing misfolded proteins by regulating autophagy cargo receptors and actin dynamics. Front Aging Neurosci. 2022;14:933979 pubmed publisher
Turturro S, Shen X, Shyam R, YUE B, Ying H. Effects of mutations and deletions in the human optineurin gene. Springerplus. 2014;3:99 pubmed publisher
product information
Catalog Number :
68848
Product Name :
pE478G-EGFP
article :
doi845 [pii]
id15593
pubmed_id24683533
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-N1
backbone_mutation
backbone_originClontech
backbone_size4733
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Wild type construct is pOPTN-EGFP (Plasmid #27052)
growth strain :
mutated OPTN was inserted into pEGFP-N1 backbone
origin :
37
pi :
alt_names
OPTN
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3EGFP-N
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesALS12, FIP2, GLC1E, HIP7, HYPL, NRP, TFIIIA-INTP
geneOPTN
id10133
genbank_ids
mutationE478G, associated with ALS
nameoptineurin
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagEGFP
resistance markers :
862
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA