pmCherryC2-Gephyrin P1 | Addgene 68820 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pmCherryC2-Gephyrin P1

catalog :

68820

citations: 3

Reference
Grupi A, Shapira Z, Yudovich S, Degani Katzav N, Weiss S. Point-localized, site-specific membrane potential optical recording by single fluorescent nanodiscs. Biophys Rep. 2021;1:None pubmed publisher
Lee J, Kim J, Noh S, Lee H, Lee S, Mun J, et al. Astrocytes phagocytose adult hippocampal synapses for circuit homeostasis. Nature. 2020;: pubmed publisher
Tyagarajan S, Ghosh H, Yevenes G, Nikonenko I, Ebeling C, Schwerdel C, et al. Regulation of GABAergic synapse formation and plasticity by GSK3beta-dependent phosphorylation of gephyrin. Proc Natl Acad Sci U S A. 2011;108:379-84 pubmed publisher

product information

Catalog Number :

68820

Product Name :

pmCherryC2-Gephyrin P1

article :

doi	10.1073/pnas.1011824108
id	15580
pubmed_id	21173228

bacterial resistance :

Kanamycin

cloning :

backbone

pEGFP C2

backbone_mutation

backbone_origin

Clontech

backbone_size

4700

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

EGFP was removed using NheI and XhoI. mCherry was amplified and inserted using NheI and XhoI sites. Gephyrin D566N mutation is a polymorphism and is not a concern for plasmid function.

growth strain :

mCherry tagged Geph for mammalian expression

origin :

pi :

alt_names

GPHN

cloning

clone_method	Restriction Enzyme
cloning_site_3	KpnI
cloning_site_5	EcoRI
promoter	CMV
sequencing_primer_3
sequencing_primer_5	EGFP-C
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	Geph
gene	Gphn
id	64845

genbank_ids

NM022865.3

mutation

silent mutations on internal EcoRI site. Gephyrin D566N (see depositor comment below)

name

Gephyrin

shRNA_sequence

size

2200

species

10116

Rattus norvegicus