This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
EGFPC2-Gephyrin P1
catalog :
68815
citations: 1
Reference
Tyagarajan S, Ghosh H, Yevenes G, Imanishi S, Zeilhofer H, Gerrits B, et al. Extracellular signal-regulated kinase and glycogen synthase kinase 3? regulate gephyrin postsynaptic aggregation and GABAergic synaptic function in a calpain-dependent mechanism. J Biol Chem. 2013;288:9634-47 pubmed publisher
product information
Catalog Number :
68815
Product Name :
EGFPC2-Gephyrin P1
article :
doi10.1074/jbc.M112.442616
id12226
pubmed_id23408424
bacterial resistance :
Kanamycin
cloning :
backbonepEGFPC2
backbone_mutation
backbone_originContech
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Gephyrin D566N mutation is a polymorphism and is not a concern for plasmid function.
growth strain :
expression of fluorescent tagged Geph in mammalian cells
origin :
37
pi :
alt_names
GPHN
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5EcoRI
promoterCMV
sequencing_primer_3SV40pA-R
sequencing_primer_5EGFP-C
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGeph
geneGphn
id64845
genbank_ids
NM022865.3
mutationsilent mutations on internal EcoRI site. Gephyrin D566N (see depositor comment below)
nameGephyrin
shRNA_sequence
size2200
species
10116
Rattus norvegicus
tags
locationN terminal on backbone
tagEGFP
resistance markers :
2515
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA