This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV-TNT-CFP-STIM1-C227W
catalog :
68403
citations: 1
Reference
Ma G, Wei M, He L, Liu C, Wu B, Zhang S, et al. Inside-out Ca(2+) signalling prompted by STIM1 conformational switch. Nat Commun. 2015;6:7826 pubmed publisher
product information
Catalog Number :
68403
Product Name :
pCMV-TNT-CFP-STIM1-C227W
article :
doi10.1038/ncomms8826
id14799
pubmed_id26184105
bacterial resistance :
Ampicillin
cloning :
backbonepCMVTnT Vector
backbone_mutation
backbone_origin
backbone_size4050
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses a STIM1 gain-of-function mutant in mammalian cells
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5EcorI
promoterCMV
sequencing_primer_3cgg GCGGCCGC CTACTTCTTAAGAGGCTTCTTAAAG
sequencing_primer_5ccg GAATTC ATGGATGTATGCGTC CGTCTTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesD11S4896E, GOK, IMD10, STRMK, TAM, TAM1
geneSTIM1
id6786
genbank_ids
mutationChanged Cysteine 227 to Tryptophan; CFP was inserted into STIM between G37 and A38.
nameCFP-STIM1
shRNA_sequence
size2781
species
9606
Homo sapiens
tags
location
tagCyan Fluorescent Protein (CFP)
resistance markers :
2581
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA