This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEGB3 Pnos:Luciferase:Tnos-SF-35S:Renilla:Tnos-35S:P19:Tnos-SF (GB1116)
catalog :
68218
citations: 1
Reference
Sarrión Perdigones A, Vazquez Vilar M, Palací J, Castelijns B, Forment J, Ziarsolo P, et al. GoldenBraid 2.0: a comprehensive DNA assembly framework for plant synthetic biology. Plant Physiol. 2013;162:1618-31 pubmed
product information
Catalog Number :
68218
Product Name :
pEGB3 Pnos:Luciferase:Tnos-SF-35S:Renilla:Tnos-35S:P19:Tnos-SF (GB1116)
article :
doi10.1104/pp.113.217661
id10316
pubmed_id23669743
bacterial resistance :
Kanamycin
cloning :
backbonepDGB3alpha1
backbone_mutation
backbone_originself-made; derived from pCambia1302 generated at the Cambia Institute
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Plant Expression
Luciferase
Synthetic Biology
growth notes :
insert can be released with BsmBI
growth strain :
Module composed of 3 transcriptional units: Luciferase and Renilla proteins, and the Tomato bushy stunt virus (TBSV) silencing suppressor P19 plant expression
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BsaI
cloning_site_5BsaI
promoterPnos / 35S / 35S
sequencing_primer_3
sequencing_primer_5GGTGGCAGGATATATTGTGG
site_3_destroyed1
site_5_destroyed1
entrez_gene
genbank_ids
mutationBsaI and BsmBI sites removed
nameLuc / Renilla / P19
shRNA_sequence
size7468
species
32644
Other
tags
resistance markers :
2490
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA