This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pTM1_VP35_ZEBOV
catalog :
68121
citations: 3
Reference
Levine C, Mire C, Geisbert T. Comparison of Zaire and Bundibugyo Ebolavirus Polymerase Complexes and Susceptibility to Antivirals through a Newly Developed Bundibugyo Minigenome System. J Virol. 2021;95:e0064321 pubmed publisher
Manhart W, Pacheco J, Hume A, Cressey T, Deflubé L, Mühlberger E. A Chimeric Lloviu Virus Minigenome System Reveals that the Bat-Derived Filovirus Replicates More Similarly to Ebolaviruses than Marburgviruses. Cell Rep. 2018;24:2573-2580.e4 pubmed publisher
Trunschke M, Conrad D, Enterlein S, Olejnik J, Brauburger K, M hlberger E. The L-VP35 and L-L interaction domains reside in the amino terminus of the Ebola virus L protein and are potential targets for antivirals. Virology. 2013;441:135-45 pubmed publisher
product information
Catalog Number :
68121
Product Name :
pTM1_VP35_ZEBOV
article :
doi10.1016/j.virol.2013.03.013
id14575
pubmed_id23582637
bacterial resistance :
Ampicillin
cloning :
backbonepTM1
backbone_mutation
backbone_originBernhard Moss, National Institutes of Health
backbone_size5357
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
T7
growth strain :
Encodes for Ebola Virus Zaire VP35 under control of a T7 RNA polymerase promoter.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5BamHI
promoterT7
sequencing_primer_3GCC AAC TCA GCT TCC TTT CGG
sequencing_primer_5ATT GTA TGG GAT CTG ATC TGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesZEBOVgp2
geneVP35
id911827
genbank_ids
mutation
nameVP35 ZEBOV
shRNA_sequence
size1415
species
tags
resistance markers :
2611
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA